Identification of Pathogenic Bacteria on Carp Commodities (Cyprinus carpio) at Quality Control and Fishery Product Safety Agency (BKIPM) of Bengkulu

https://doi.org/10.47194/orics.v1i2.72

Authors

Keywords:

Cyprinus carpio, Aeromonas hydrophila, Plesiomonas shigelloides

Abstract

Carp (Cyprinus carpio) is a type of freshwater fish that is widely cultivated. The increase in the amount of production and trade in freshwater fishery commodities both for consumption in Bengkulu will potentially increase the risk of entry and spread of pests and diseases in fish, which at the same time will be a threat that can endanger and damage the sustainability of fishery biological resources. Bacteria that infect fish can inhibit the expected production targets, which is an outbreak of pathogenic fish disease caused by bacteria. This study aims to identify pathogenic bacteria that infect Carp (C. carpio). Carp were obtained from fish traders at Panorama Market, Bengkulu City. Carp samples were selected based on clinical symptoms that were no longer healthy. Isolation of bacteria from Carp’s organs using Triptic Soy Agar (TSA) media. The isolates were screened by morphological characters and biochemical test. The results of this study showed that total of 2 bacteria were isolated. Based on biochemical tests carried out such as the Simmons Citrate test, Triple Sugar Iron Agar, Oxidative-Fermentative, Motility Indol Ornithine, Lysine Iron Agar, MR-VP, urea, catalase, oxidase, gelatin, confectionery test, and Rimmler-Shotts test, pathogenic isolates Sp 1. in the sample have a close relationship with Plesiomonas shigelloides while the pathogenic isolates Sp 2. and Sp 3. have a close relationship with Aeromonas hydrophila.

References

Anggraini, R., Aliza, D., & Mellisa, S. (2016). Identifikasi Bakteri Aeromonas hydrophila dengan Uji Mikrobiologi pada Ikan Lele Dumbo (Clarias gariepinus) yang Dibudidayakan di Kecamatan Baitussalam Kabupaten Aceh Besar. Jurnal Ilmiah Mahasiswa Kelautan dan Perikanan Unsyiah, 1, 271-286.

Behera, B. K., Bera, A. K., Paria, P., Das, A., Parida, P. K., Kumari, S., & Bhowmick, S. (2018). Identification and pathogenicity of Plesiomonas shigelloides in Silver Carp. Journal of aquaculture, 4, 314–318.

Chandra, T. J., & Mani, S. (2011). A study of 2 Rapid Tests to Differentiate Gram Positive and Gram Negative Aerobic Bacteria. Journal of Medical and Allied Sciences, 1, 84-85.

Cowan, & Steel. (1993). Manual for The Identification of Medical Bacteria, Third Edition, Cambridge University Press, United Kingdom.

Hemraj, V., Diksha, S., & Avneet, G. (2013). A Review on Commonly used Biochemical Test for Bacteria. Innofare journal of life science, 1, 1-7.

Murwantoko, Rozi, Istiqomah. I., & Kamiso, H. N. (2013). Isolasi, Karakterisasi, dan Patogenitas Bakteri Penyebab Penyakit Pada Gurami (Osphronemus Goramy) Di Kabupaten Bantul. J. Fish Scie, 15, 83-90.

Pratama, R. C., Rosidah, Sriati, & Ike, R. (2017). Efektifitas Ektrak Biji Rambutan dalam Mengobati Benih Ikan Mas yang Terinfeksi Bakteri Aeromonas hydrophila. Jurnal Perikanan Dan Kelautan, 7(1), 130-138.

Purnamawati, R. (2016). Fish Disease Inspection Methods at Fish Quarantine Center for Quality and Safety Control of Class I Fishery Products in Surabaya. Skripsi, Undergraduate Program of Aquaculture, University of Airlangga.

Putra, A. N., Ningsih, C. W., Nurani, F. S., Mustahal, M., & Indaryanto, F. R. (2018). Evaluation of Fermentation on Moringa Leaves (Moringa oleifera) as Raw Material on Feed of Tilapia (Oreochromis niloticus). Jurnal Perikanan dan Kelautan, 8(2), 104-113.

Rondonuwu, G., Kepel, B. J., & Bodhi, W. (2014). Gambaran Bakteri Resistensi HgCl2 Dan Fenil Merkuri yang Diambil dari Feses, Urin, dan Karang Gigi pada Individu yang Tinggal di Daerah Pesisir Pantai di Desa Kema II. Jurnal e-Biomedik (eBM), 2, 1-10.

Septiama, Sugiarti, B., Aritonang. A., Novel, D., Astri, A., Noor, R., Wahyuni, I., Hapsari, I., Fakriza, I., David, P., Gunardi, A., & Aryati, E. (2010). Method of Pest Inspection of Quarantined Fish Diseases for Aeromonas salmonicida Bacteria, PUSKARI, Jakarta.

Tantu, W., Reiny, A. T., & Sammy, N. J. L. (2013). Detection of the Presence Aeromonas sp on Nile Tilapia Cultured in Floating Net Cage in Lake Tondano. J. Budidaya Perairan, 1, 74-80.

Ulfa, Endang, S., & Mimien, H. (2016). Isolasi dan Uji Sensitivitas Merkuri pada Bakteri dari Limbah Penambangan Emas di Sekotong barat Kabupaten Lombok Barat: Penelitian Pendahuluan. Proceeding Biology Education Conference (ISSN: 2528-5742), 13(1), 12-24.

Yulvizar, Risa, N., & Agustiana, D. C. (2014). Isolasi dan Karakterisasi Bakteri pada Ikan Kembung (Rastrelliger sp.) Asin Berkitosan. J. Biospecies, 6, 15-19.

Published

2020-06-03

How to Cite

Wibowo, R. H. (2020). Identification of Pathogenic Bacteria on Carp Commodities (Cyprinus carpio) at Quality Control and Fishery Product Safety Agency (BKIPM) of Bengkulu. Operations Research: International Conference Series, 1(2), 50–55. https://doi.org/10.47194/orics.v1i2.72